@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://opendata.teagasc.ie/dataset/ab5c94fe-4fb5-4140-8479-6f7da7645010> a dcat:Dataset ;
    dct:identifier "ab5c94fe-4fb5-4140-8479-6f7da7645010" ;
    dct:issued "2026-10-02T15:44:19"^^xsd:dateTime ;
    dct:language "en" ;
    dct:modified "2026-10-02T15:52:27"^^xsd:dateTime ;
    dct:provenance "Colostrum was collected from each quarter within 2-6 h of calving using a portable milking unit, following sterile microbiome sampling procedures (equipment cleaned with antimicrobial soap and boiling water, teats and clusters disinfected with 70% ethanol). Each donor's colostrum was stored separately in sterile buckets at 4 C for no more than 24 h, combined with a second donor where needed, and reheated to 38 C in a water bath for 60 min. Calves (n = 21) did not receive colostrum from their own dam and were fed 8.5% of birthweight within 2 h of birth. Immediately before feeding, 30 mL was collected aseptically into three sterile 10 mL tubes, snap frozen in liquid nitrogen and stored at -80 C.   Samples were thawed stepwise (-20 C for 24 h, then 4 C for 24 h), treated with EDTA and centrifuged (4,500 x g, 20 min, 4 C). The fat layer was discarded, the supernatant kept for sRID assays, and the microbial pellet used for DNA extraction (Qiagen DNeasy PowerSoil Pro Kit, repeated bead beating). Bacterial DNA presence was confirmed by qPCR (Kittelmann et al. 2013 method). Library preparation and sequencing were performed by Macrogen (Seoul, South Korea): V4 region amplified with 515F/806R primers carrying Nextera overhang adapters, 25 PCR cycles, AMPure purification, sequenced on an Illumina MiSeq with the 500-cycle v2 reagent kit on one flow cell.   Herd management: cows and heifers grazed perennial ryegrass/white clover until November, then were housed on grass silage ad libitum without concentrate. Dry-off treatments included parasite control and teat sealant for multiparous cows; 11 multiparous cows with SCC > 100,000 also received intramammary antibiotic.   Full protocols: Scully et al. (2026) PLOS ONE 21(7), e0353693, and Scully et al. (2025) PLOS ONE 20(10), e0335718." ;
    dct:publisher <https://opendata.teagasc.ie/organization/b16b4c0b-15bd-4654-b227-792210c7076b> ;
    dct:relation "Scully, S., Earley, B., Smith, P. E., Finnie, M. S. J., McAloon, C., Kenny, D. A. & Waters, S. M. (2026). Characterisation of the bacterial and archaeal microbiota in processed colostrum collected from a spring-calving dairy herd. PLOS ONE 21(7), e0353693. https://doi.org/10.1371/journal.pone.0353693" ;
    dct:title "Bovine Colostrum Raw sequence reads" ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "Dr David Kenny" ;
            vcard:hasEmail <mailto:david.kenny@teagasc.ie> ] ;
    dcat:keyword "Colostrum",
        "Dairy farming",
        "Gene sequencing",
        "Microbiome" ;
    dcat:landingPage <https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1136803/> ;
    dcat:theme <animal_and_grassland> .

<https://opendata.teagasc.ie/organization/b16b4c0b-15bd-4654-b227-792210c7076b> a foaf:Organization ;
    foaf:name "Animal and Bioscience Research" .