@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://opendata.teagasc.ie/dataset/abef3c2a-2737-4b4a-85d3-33ae9c22b9e2> a dcat:Dataset ;
    dct:identifier "abef3c2a-2737-4b4a-85d3-33ae9c22b9e2" ;
    dct:issued "2026-09-29T15:40:20"^^xsd:dateTime ;
    dct:language "en" ;
    dct:modified "2026-09-29T16:08:51"^^xsd:dateTime ;
    dct:provenance "Raw read quality was checked with FASTQC; adapters and low-quality reads were removed with Cutadapt. Trimmed reads were aligned to the bovine reference genome ARS-UCD1.2 with STAR (mean 90.62% mapped), and gene-level counts for protein-coding genes were generated using STAR \"quantMode GeneCounts\" against the Ensembl v87 annotation. Genes with <1 count per million in at least half the samples per comparison were removed; data were TMM-normalised and differential expression between H- and L-RFI steers was tested within each breed x diet comparison using exact tests in edgeR. A separate model evaluated the breed x diet interaction (RFI as class variable, animal as random effect). DEGs were defined as Benjamini-Hochberg FDR < 0.1 and fold change > 1.5 (230 DEGs across six comparisons; 23 interaction genes). DEGs were annotated and analysed for pathway enrichment using Ingenuity Pathway Analysis (Qiagen)." ;
    dct:publisher <https://opendata.teagasc.ie/organization/b16b4c0b-15bd-4654-b227-792210c7076b> ;
    dct:relation "Keogh, K., McKenna, C., Waters, S. M., Porter, R. K., Fitzsimons, C., McGee, M. & Kenny, D. A. (2023). Effect of breed and diet on the M. longissimus thoracis et lumborum transcriptome of steers divergent for residual feed intake. Scientific Reports 13, 9034. https://doi.org/10.1038/s41598-023-35661-z" ;
    dct:title "The effect of diet and breed on global gene expression profiles of animals divergent for RFI" ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "Dr David Kenny" ;
            vcard:hasEmail <mailto:david.kenny@teagasc.ie> ] ;
    dcat:keyword "Feed efficiency",
        "RNA-seq",
        "Residual feed intake",
        "beef cattle",
        "skeletal muscle" ;
    dcat:landingPage <https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE113135> ;
    dcat:theme <animal_and_grassland> .

<https://opendata.teagasc.ie/organization/b16b4c0b-15bd-4654-b227-792210c7076b> a foaf:Organization ;
    foaf:name "Animal and Bioscience Research" .

