Data and Resources




This dataset has no data

Additional Info

Field Value
Title Fungal microbiome structure across phyllosphere compartments of Eucalyptus cinerea
License Licence Not Specified
Teagasc Department Forestry Development
Teagasc Programme Crops, Environment and Land Use
Description Fungal ITS amplicon sequencing data (Illumina NovaSeq, 2 x 150 bp) from leaf, stem and bark tissues of five Eucalyptus cinerea trees in an intensively managed commercial cut foliage plantation in Co. Dublin, sampled on one day in July 2024. Tissues were pooled by compartment within each tree, giving 15 samples (5 trees x 3 compartments). Tissues were not surface-sterilised, so the profiles combine epiphytic and endophytic fungi. After quality control and DADA2 denoising, 405 fungal ASVs were retained. Bark carried richer, more diverse and phylogenetically broader fungal communities than leaf and stem: bark was dominated by Diaporthe, Peniophora and Talaromyces, and leaf and stem by Vishniacozyma and Sporobolomyces. Both compartment (PERMANOVA R2 = 0.239) and tree identity (R2 = 0.451) significantly affected community composition. The dataset is a baseline description of the fungal microbiome of E. cinerea grown commercially under Irish conditions.
Language English
Principal Investigator (PI) Dr Dheeraj Singh Rathore
Principal Investigator (PI) email dheeraj.rathore@teagasc.ie
Principal Investigator (PI) ORCID https://orcid.org/0000-0003-4931-7263
Data creator(s)
  1. Tomás Byrne
Landing page https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1460758/
Related resources
  1. Byrne, T. & Rathore, D. S. (2026). Fungal Microbiome Structure Across Phyllosphere Compartments in Intensively Managed Eucalyptus cinerea for Cut Foliage Production. Applied Microbiology 6(7), 76. https://doi.org/10.3390/applmicrobiol6070076
  2. Bioinformatic workflows and analysis scripts are available on GitHub at: https://github.com/ByrneTforestry/its-fungal-amplicon-Eucalyptus
Provenance information A commercial E. cinerea cut foliage plantation in Co. Dublin, established in 2019 and managed intensively (irrigation, fertilisation, herbicide weed control). Five trees of similar age and management history were selected at random across three production fields. From each tree, six leaves, six stem sections (about 2 cm) and six bark pieces were taken from several canopy positions and pooled by compartment. All samples were collected on one day, put on ice, and stored at -80 C. Tissues were not surface-sterilised. DNA and sequencing: total DNA was extracted with the Qiagen DNeasy Plant Mini Kit. Novogene (Cambridge, UK) amplified the fungal ITS region with primers ITS5-1737F (5'-GGAAGTAAAAGTCGTAACAAGG-3') and ITS2-2043R (5'-GCTGGTTCATTCATCGATGC-3'), prepared libraries, and sequenced them on an Illumina NovaSeq (PE150). Library negative controls produced no detectable amplification and were not sequenced.
Time of data collection July 2024