Data and Resources

This dataset has no data

• Eucalyptus cinerea
• Foliage
• Mycobiome
• Phyllosphere microb...
• Sequencing

Additional Info

Field	Value
Title	Fungal microbiome structure across phyllosphere compartments of Eucalyptus cinerea
License	Licence Not Specified
Teagasc Department	Forestry Development
Téama	Crops, Environment and Land Use
Cur síos
Language	English
Principal Investigator (PI)	Dr Dheeraj Singh Rathore
Principal Investigator (PI) email	dheeraj.rathore@teagasc.ie
Principal Investigator (PI) ORCID	https://orcid.org/0000-0003-4931-7263
Data creator(s)	Tomás Byrne
Landing page	https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1460758/
Related resources	Byrne, T. & Rathore, D. S. (2026). Fungal Microbiome Structure Across Phyllosphere Compartments in Intensively Managed Eucalyptus cinerea for Cut Foliage Production. Applied Microbiology 6(7), 76. https://doi.org/10.3390/applmicrobiol6070076 Bioinformatic workflows and analysis scripts are available on GitHub at: https://github.com/ByrneTforestry/its-fungal-amplicon-Eucalyptus
Provenance information	A commercial E. cinerea cut foliage plantation in Co. Dublin, established in 2019 and managed intensively (irrigation, fertilisation, herbicide weed control). Five trees of similar age and management history were selected at random across three production fields. From each tree, six leaves, six stem sections (about 2 cm) and six bark pieces were taken from several canopy positions and pooled by compartment. All samples were collected on one day, put on ice, and stored at -80 C. Tissues were not surface-sterilised. DNA and sequencing: total DNA was extracted with the Qiagen DNeasy Plant Mini Kit. Novogene (Cambridge, UK) amplified the fungal ITS region with primers ITS5-1737F (5'-GGAAGTAAAAGTCGTAACAAGG-3') and ITS2-2043R (5'-GCTGGTTCATTCATCGATGC-3'), prepared libraries, and sequenced them on an Illumina NovaSeq (PE150). Library negative controls produced no detectable amplification and were not sequenced.
Time of data collection	July 2024