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On 8 October 2026 at 08:28:07 UTC,
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| f | 1 | { | f | 1 | { |
| 2 | "api_access_url": "", | 2 | "api_access_url": "", | ||
| 3 | "api_response_formats": [], | 3 | "api_response_formats": [], | ||
| 4 | "api_type": "", | 4 | "api_type": "", | ||
| 5 | "author": null, | 5 | "author": null, | ||
| 6 | "author_email": null, | 6 | "author_email": null, | ||
| 7 | "citation": "Vexler, L., Byrne, S., & Milbourne, D. (2026). k-mer | 7 | "citation": "Vexler, L., Byrne, S., & Milbourne, D. (2026). k-mer | ||
| 8 | analysis of publicly available resources enables rapid development of | 8 | analysis of publicly available resources enables rapid development of | ||
| 9 | an improved KASP marker linked to the H1 resistance locus in potato | 9 | an improved KASP marker linked to the H1 resistance locus in potato | ||
| 10 | [Dataset]. Zenodo. https://doi.org/10.5281/zenodo.19629845", | 10 | [Dataset]. Zenodo. https://doi.org/10.5281/zenodo.19629845", | ||
| 11 | "commercial_data": "false", | 11 | "commercial_data": "false", | ||
| 12 | "conforms_to": "", | 12 | "conforms_to": "", | ||
| 13 | "contact_email": "stephen.byrne@teagasc.ie", | 13 | "contact_email": "stephen.byrne@teagasc.ie", | ||
| 14 | "contact_name": "Stephen Byrne ", | 14 | "contact_name": "Stephen Byrne ", | ||
| 15 | "creator_user_id": "68d970d0-ff26-4dfe-ab51-46dde87e3f23", | 15 | "creator_user_id": "68d970d0-ff26-4dfe-ab51-46dde87e3f23", | ||
| 16 | "data_creator": [ | 16 | "data_creator": [ | ||
| 17 | "Vexler, L", | 17 | "Vexler, L", | ||
| 18 | "Milbourne, D." | 18 | "Milbourne, D." | ||
| 19 | ], | 19 | ], | ||
| 20 | "doi": "https://doi.org/10.5281/zenodo.19629845", | 20 | "doi": "https://doi.org/10.5281/zenodo.19629845", | ||
| 21 | "equipment_used": "", | 21 | "equipment_used": "", | ||
| 22 | "funding_acknowledgment": "European Union Horizon Europe, IPMorama | 22 | "funding_acknowledgment": "European Union Horizon Europe, IPMorama | ||
| 23 | project (\"Integrating breeding for IPM into the deployment landscape | 23 | project (\"Integrating breeding for IPM into the deployment landscape | ||
| 24 | for wheat, potatoes and grain legumes\"), grant agreement 101135348", | 24 | for wheat, potatoes and grain legumes\"), grant agreement 101135348", | ||
| 25 | "geographic_coverage": "Teagasc Oak Park, Carlow, Ireland", | 25 | "geographic_coverage": "Teagasc Oak Park, Carlow, Ireland", | ||
| 26 | "groups": [], | 26 | "groups": [], | ||
| 27 | "high_value_data": "false", | 27 | "high_value_data": "false", | ||
| 28 | "id": "643b11d9-a4f7-4a63-8b2d-c6a4c4e071bd", | 28 | "id": "643b11d9-a4f7-4a63-8b2d-c6a4c4e071bd", | ||
| 29 | "if_not_open_why": "", | 29 | "if_not_open_why": "", | ||
| 30 | "isopen": true, | 30 | "isopen": true, | ||
| 31 | "language": "en", | 31 | "language": "en", | ||
| 32 | "license_id": "CC-BY-4.0", | 32 | "license_id": "CC-BY-4.0", | ||
| 33 | "license_title": "Creative Commons Attribution 4.0", | 33 | "license_title": "Creative Commons Attribution 4.0", | ||
| 34 | "license_url": "https://creativecommons.org/licenses/by/4.0/", | 34 | "license_url": "https://creativecommons.org/licenses/by/4.0/", | ||
| 35 | "maintainer": null, | 35 | "maintainer": null, | ||
| 36 | "maintainer_email": null, | 36 | "maintainer_email": null, | ||
| 37 | "metadata_created": "2026-10-07T15:31:23.391545", | 37 | "metadata_created": "2026-10-07T15:31:23.391545", | ||
| n | 38 | "metadata_modified": "2026-10-07T15:31:23.391551", | n | 38 | "metadata_modified": "2026-10-08T08:28:07.436006", |
| 39 | "name": | 39 | "name": | ||
| 40 | arker-development-for-potato-h1-resistance-via-public-k-mer-analysis", | 40 | arker-development-for-potato-h1-resistance-via-public-k-mer-analysis", | ||
| 41 | "notes_translated": { | 41 | "notes_translated": { | ||
| 42 | "en": "Electronic supplementary material (ESM S1-S5) for a study | 42 | "en": "Electronic supplementary material (ESM S1-S5) for a study | ||
| 43 | that redeveloped a KASP marker for the H1 potato cyst nematode (PCN) | 43 | that redeveloped a KASP marker for the H1 potato cyst nematode (PCN) | ||
| 44 | resistance locus in potato. Bulked resequencing data from Meade et al. | 44 | resistance locus in potato. Bulked resequencing data from Meade et al. | ||
| 45 | (2020) were re-analysed with the CoSSA k-mer subtraction approach. | 45 | (2020) were re-analysed with the CoSSA k-mer subtraction approach. | ||
| 46 | H1-associated k-mers were extracted and mapped to the DM potato | 46 | H1-associated k-mers were extracted and mapped to the DM potato | ||
| 47 | reference genome, giving 99 candidate SNPs near the diagnostic 57R | 47 | reference genome, giving 99 candidate SNPs near the diagnostic 57R | ||
| 48 | marker region. Two SNPs were converted to KASP assays and tested on | 48 | marker region. Two SNPs were converted to KASP assays and tested on | ||
| 49 | three years of breeding material. One gave a modest but statistically | 49 | three years of breeding material. One gave a modest but statistically | ||
| 50 | significant improvement in classification accuracy over the previously | 50 | significant improvement in classification accuracy over the previously | ||
| 51 | published marker. The dataset documents a low-cost workflow for | 51 | published marker. The dataset documents a low-cost workflow for | ||
| 52 | turning reference-free k-mer signals from public sequence data into | 52 | turning reference-free k-mer signals from public sequence data into | ||
| 53 | breeder-ready marker assays. It contains: the k-mer datasets and set | 53 | breeder-ready marker assays. It contains: the k-mer datasets and set | ||
| 54 | operations used (S1); the effect of k-mer depth thresholds on | 54 | operations used (S1); the effect of k-mer depth thresholds on | ||
| 55 | genome-wide signal and H1 enrichment (S2, S3); multi-year KASP calls | 55 | genome-wide signal and H1 enrichment (S2, S3); multi-year KASP calls | ||
| 56 | with phenotypic PCN resistance data for the validation panel (S4); and | 56 | with phenotypic PCN resistance data for the validation panel (S4); and | ||
| 57 | the 99 candidate SNPs (S5).", | 57 | the 99 candidate SNPs (S5).", | ||
| 58 | "ga": "" | 58 | "ga": "" | ||
| 59 | }, | 59 | }, | ||
| 60 | "num_resources": 0, | 60 | "num_resources": 0, | ||
| 61 | "num_tags": 4, | 61 | "num_tags": 4, | ||
| 62 | "open_data": "Open data", | 62 | "open_data": "Open data", | ||
| 63 | "orcid_id": "http://orcid.org/0000-0002-1179-2272", | 63 | "orcid_id": "http://orcid.org/0000-0002-1179-2272", | ||
| 64 | "organization": { | 64 | "organization": { | ||
| 65 | "approval_status": "approved", | 65 | "approval_status": "approved", | ||
| 66 | "created": "2025-09-02T11:44:16.121764", | 66 | "created": "2025-09-02T11:44:16.121764", | ||
| 67 | "description": "", | 67 | "description": "", | ||
| 68 | "id": "9cf31806-11fa-422c-b1d4-a68a52abd204", | 68 | "id": "9cf31806-11fa-422c-b1d4-a68a52abd204", | ||
| 69 | "image_url": "", | 69 | "image_url": "", | ||
| 70 | "is_organization": true, | 70 | "is_organization": true, | ||
| 71 | "name": "crops-research", | 71 | "name": "crops-research", | ||
| 72 | "state": "active", | 72 | "state": "active", | ||
| 73 | "title": "Crops Research", | 73 | "title": "Crops Research", | ||
| 74 | "type": "organization" | 74 | "type": "organization" | ||
| 75 | }, | 75 | }, | ||
| 76 | "owner_org": "9cf31806-11fa-422c-b1d4-a68a52abd204", | 76 | "owner_org": "9cf31806-11fa-422c-b1d4-a68a52abd204", | ||
| 77 | "personal_data_flag": "false", | 77 | "personal_data_flag": "false", | ||
| 78 | "personal_sensitive_data": "false", | 78 | "personal_sensitive_data": "false", | ||
| 79 | "private": false, | 79 | "private": false, | ||
| 80 | "provenance": "Publicly available bulked resequencing data from | 80 | "provenance": "Publicly available bulked resequencing data from | ||
| 81 | Meade et al. (2020) were downloaded from the read archives (ESM S1). | 81 | Meade et al. (2020) were downloaded from the read archives (ESM S1). | ||
| 82 | The new KASP assays were validated on potato breeding material across | 82 | The new KASP assays were validated on potato breeding material across | ||
| 83 | three years, with PCN resistance phenotypes. k-mers were counted in | 83 | three years, with PCN resistance phenotypes. k-mers were counted in | ||
| 84 | resistant and susceptible sequencing pools, and H1-associated k-mers | 84 | resistant and susceptible sequencing pools, and H1-associated k-mers | ||
| 85 | extracted by set subtraction (CoSSA), testing several depth thresholds | 85 | extracted by set subtraction (CoSSA), testing several depth thresholds | ||
| 86 | (ESM S2, S3). The retained k-mers were mapped to the DM reference | 86 | (ESM S2, S3). The retained k-mers were mapped to the DM reference | ||
| 87 | genome to identify candidate SNPs near the 57R region (ESM S5). Two | 87 | genome to identify candidate SNPs near the 57R region (ESM S5). Two | ||
| 88 | SNPs were converted to KASP assays and their classification accuracy | 88 | SNPs were converted to KASP assays and their classification accuracy | ||
| 89 | compared with the previously published marker against phenotypic PCN | 89 | compared with the previously published marker against phenotypic PCN | ||
| 90 | resistance (ESM S4)", | 90 | resistance (ESM S4)", | ||
| 91 | "publish_to_public": true, | 91 | "publish_to_public": true, | ||
| 92 | "related_resource": [ | 92 | "related_resource": [ | ||
| 93 | "Vexler L, Clot CR, Byrne S et al. k-mer analysis of publicly | 93 | "Vexler L, Clot CR, Byrne S et al. k-mer analysis of publicly | ||
| 94 | available resources enables rapid development of an improved KASP | 94 | available resources enables rapid development of an improved KASP | ||
| 95 | marker linked to the H1 resistance locus in potato [version 1; peer | 95 | marker linked to the H1 resistance locus in potato [version 1; peer | ||
| 96 | review: awaiting peer review]. Open Res Europe 2026, 6:342 | 96 | review: awaiting peer review]. Open Res Europe 2026, 6:342 | ||
| 97 | (https://doi.org/10.12688/openreseurope.23753.1)", | 97 | (https://doi.org/10.12688/openreseurope.23753.1)", | ||
| 98 | "Meade, F., Byrne, S., Griffin, D. et al. Rapid Development of | 98 | "Meade, F., Byrne, S., Griffin, D. et al. Rapid Development of | ||
| 99 | KASP Markers for Disease Resistance Genes Using Pooled Whole-Genome | 99 | KASP Markers for Disease Resistance Genes Using Pooled Whole-Genome | ||
| 100 | Resequencing. Potato Res. 63, 57\u201373 (2020). | 100 | Resequencing. Potato Res. 63, 57\u201373 (2020). | ||
| 101 | https://doi.org/10.1007/s11540-019-09428-x", | 101 | https://doi.org/10.1007/s11540-019-09428-x", | ||
| 102 | "Zenodo record 19629845, Version v1, published 17 April 2026, in | 102 | "Zenodo record 19629845, Version v1, published 17 April 2026, in | ||
| 103 | the IPMorama community: https://doi.org/10.5281/zenodo.19629845" | 103 | the IPMorama community: https://doi.org/10.5281/zenodo.19629845" | ||
| 104 | ], | 104 | ], | ||
| 105 | "relationships_as_object": [], | 105 | "relationships_as_object": [], | ||
| 106 | "relationships_as_subject": [], | 106 | "relationships_as_subject": [], | ||
| 107 | "resources": [], | 107 | "resources": [], | ||
| 108 | "rights": "https://creativecommons.org/licenses/by/4.0/deed.en", | 108 | "rights": "https://creativecommons.org/licenses/by/4.0/deed.en", | ||
| 109 | "spatial": "", | 109 | "spatial": "", | ||
| 110 | "spatial_resolution": "", | 110 | "spatial_resolution": "", | ||
| 111 | "spatial_uri": "", | 111 | "spatial_uri": "", | ||
| 112 | "srs": [], | 112 | "srs": [], | ||
| t | 113 | "state": "approval_needed", | t | 113 | "state": "active", |
| 114 | "supplementary_data": "", | 114 | "supplementary_data": "", | ||
| 115 | "tags": [ | 115 | "tags": [ | ||
| 116 | { | 116 | { | ||
| 117 | "display_name": "K-mer analysis", | 117 | "display_name": "K-mer analysis", | ||
| 118 | "id": "48f1ca86-9bd5-4897-b48f-ad2ea17db3d9", | 118 | "id": "48f1ca86-9bd5-4897-b48f-ad2ea17db3d9", | ||
| 119 | "name": "K-mer analysis", | 119 | "name": "K-mer analysis", | ||
| 120 | "state": "active", | 120 | "state": "active", | ||
| 121 | "vocabulary_id": null | 121 | "vocabulary_id": null | ||
| 122 | }, | 122 | }, | ||
| 123 | { | 123 | { | ||
| 124 | "display_name": "Molecular markers", | 124 | "display_name": "Molecular markers", | ||
| 125 | "id": "1c0a0a64-0649-4873-9563-77ad1fef172f", | 125 | "id": "1c0a0a64-0649-4873-9563-77ad1fef172f", | ||
| 126 | "name": "Molecular markers", | 126 | "name": "Molecular markers", | ||
| 127 | "state": "active", | 127 | "state": "active", | ||
| 128 | "vocabulary_id": null | 128 | "vocabulary_id": null | ||
| 129 | }, | 129 | }, | ||
| 130 | { | 130 | { | ||
| 131 | "display_name": "Potato", | 131 | "display_name": "Potato", | ||
| 132 | "id": "5de0d9de-331e-402d-b8dc-79e314862ba1", | 132 | "id": "5de0d9de-331e-402d-b8dc-79e314862ba1", | ||
| 133 | "name": "Potato", | 133 | "name": "Potato", | ||
| 134 | "state": "active", | 134 | "state": "active", | ||
| 135 | "vocabulary_id": null | 135 | "vocabulary_id": null | ||
| 136 | }, | 136 | }, | ||
| 137 | { | 137 | { | ||
| 138 | "display_name": "Solanum tuberosum", | 138 | "display_name": "Solanum tuberosum", | ||
| 139 | "id": "fc26d31b-4310-4ca1-8a87-67f6fe1cabb5", | 139 | "id": "fc26d31b-4310-4ca1-8a87-67f6fe1cabb5", | ||
| 140 | "name": "Solanum tuberosum", | 140 | "name": "Solanum tuberosum", | ||
| 141 | "state": "active", | 141 | "state": "active", | ||
| 142 | "vocabulary_id": null | 142 | "vocabulary_id": null | ||
| 143 | } | 143 | } | ||
| 144 | ], | 144 | ], | ||
| 145 | "temporal": "", | 145 | "temporal": "", | ||
| 146 | "theme": "crops_environment_and_land_use", | 146 | "theme": "crops_environment_and_land_use", | ||
| 147 | "title": "KASP Marker Development for Potato H1 Resistance via | 147 | "title": "KASP Marker Development for Potato H1 Resistance via | ||
| 148 | Public K-mer Analysis", | 148 | Public K-mer Analysis", | ||
| 149 | "title_translated": { | 149 | "title_translated": { | ||
| 150 | "en": "KASP Marker Development for Potato H1 Resistance via Public | 150 | "en": "KASP Marker Development for Potato H1 Resistance via Public | ||
| 151 | K-mer Analysis", | 151 | K-mer Analysis", | ||
| 152 | "ga": "" | 152 | "ga": "" | ||
| 153 | }, | 153 | }, | ||
| 154 | "type": "dataset", | 154 | "type": "dataset", | ||
| 155 | "update_frequency": "", | 155 | "update_frequency": "", | ||
| 156 | "url": "https://zenodo.org/records/19629845", | 156 | "url": "https://zenodo.org/records/19629845", | ||
| 157 | "version": "", | 157 | "version": "", | ||
| 158 | "vertical_extent": "" | 158 | "vertical_extent": "" | ||
| 159 | } | 159 | } |
