Data and Resources

This dataset has no data

• K-mer analysis
• Molecular markers
• Potato
• Solanum tuberosum

Additional Info

Field	Value
Title	KASP Marker Development for Potato H1 Resistance via Public K-mer Analysis
License	Creative Commons Attribution 4.0
Teagasc Department	Crops Research
Téama	Crops, Environment and Land Use
Cur síos
Language	English
Principal Investigator (PI)	Stephen Byrne
Principal Investigator (PI) email	stephen.byrne@teagasc.ie
Principal Investigator (PI) ORCID	http://orcid.org/0000-0002-1179-2272
Data creator(s)	Vexler, L Milbourne, D.
Geographic coverage	Teagasc Oak Park, Carlow, Ireland
Digital Object Identifier (DOI)	https://doi.org/10.5281/zenodo.19629845
Citation	Vexler, L., Byrne, S., & Milbourne, D. (2026). k-mer analysis of publicly available resources enables rapid development of an improved KASP marker linked to the H1 resistance locus in potato [Dataset]. Zenodo. https://doi.org/10.5281/zenodo.19629845
Landing page	https://zenodo.org/records/19629845
Rights notes	https://creativecommons.org/licenses/by/4.0/deed.en
Project funders	European Union Horizon Europe, IPMorama project ("Integrating breeding for IPM into the deployment landscape for wheat, potatoes and grain legumes"), grant agreement 101135348
Related resources	Vexler L, Clot CR, Byrne S et al. k-mer analysis of publicly available resources enables rapid development of an improved KASP marker linked to the H1 resistance locus in potato [version 1; peer review: awaiting peer review]. Open Res Europe 2026, 6:342 (https://doi.org/10.12688/openreseurope.23753.1) Meade, F., Byrne, S., Griffin, D. et al. Rapid Development of KASP Markers for Disease Resistance Genes Using Pooled Whole-Genome Resequencing. Potato Res. 63, 57–73 (2020). https://doi.org/10.1007/s11540-019-09428-x Zenodo record 19629845, Version v1, published 17 April 2026, in the IPMorama community: https://doi.org/10.5281/zenodo.19629845
Provenance information	Publicly available bulked resequencing data from Meade et al. (2020) were downloaded from the read archives (ESM S1). The new KASP assays were validated on potato breeding material across three years, with PCN resistance phenotypes. k-mers were counted in resistant and susceptible sequencing pools, and H1-associated k-mers extracted by set subtraction (CoSSA), testing several depth thresholds (ESM S2, S3). The retained k-mers were mapped to the DM reference genome to identify candidate SNPs near the 57R region (ESM S5). Two SNPs were converted to KASP assays and their classification accuracy compared with the previously published marker against phenotypic PCN resistance (ESM S4)