Changes
On 2 October 2026 at 15:52:27 UTC,
-
No fields were updated. See the metadata diff for more details.
| f | 1 | { | f | 1 | { |
| 2 | "api_access_url": "", | 2 | "api_access_url": "", | ||
| 3 | "api_response_formats": [], | 3 | "api_response_formats": [], | ||
| 4 | "api_type": "", | 4 | "api_type": "", | ||
| 5 | "author": null, | 5 | "author": null, | ||
| 6 | "author_email": null, | 6 | "author_email": null, | ||
| 7 | "citation": "", | 7 | "citation": "", | ||
| 8 | "commercial_data": "false", | 8 | "commercial_data": "false", | ||
| 9 | "conforms_to": "", | 9 | "conforms_to": "", | ||
| 10 | "contact_email": "david.kenny@teagasc.ie", | 10 | "contact_email": "david.kenny@teagasc.ie", | ||
| 11 | "contact_name": "Dr David Kenny", | 11 | "contact_name": "Dr David Kenny", | ||
| 12 | "creator_user_id": "68d970d0-ff26-4dfe-ab51-46dde87e3f23", | 12 | "creator_user_id": "68d970d0-ff26-4dfe-ab51-46dde87e3f23", | ||
| 13 | "data_creator": [ | 13 | "data_creator": [ | ||
| 14 | "Sabine Scully", | 14 | "Sabine Scully", | ||
| 15 | "Bernadette Earley", | 15 | "Bernadette Earley", | ||
| 16 | "Paul E. Smith", | 16 | "Paul E. Smith", | ||
| 17 | "Matthew S. J. Finnie", | 17 | "Matthew S. J. Finnie", | ||
| 18 | "Catherine McAloon" | 18 | "Catherine McAloon" | ||
| 19 | ], | 19 | ], | ||
| 20 | "doi": "", | 20 | "doi": "", | ||
| 21 | "equipment_used": "", | 21 | "equipment_used": "", | ||
| 22 | "funding_acknowledgment": "European Union Horizon 2020, | 22 | "funding_acknowledgment": "European Union Horizon 2020, | ||
| 23 | \"HoloRuminant\" project (grant agreement no. 101000213)", | 23 | \"HoloRuminant\" project (grant agreement no. 101000213)", | ||
| 24 | "geographic_coverage": "Dairygold Research Farm, Kilworth, Co. Cork, | 24 | "geographic_coverage": "Dairygold Research Farm, Kilworth, Co. Cork, | ||
| 25 | Ireland (Teagasc Animal and Grassland Research and Innovation Centre, | 25 | Ireland (Teagasc Animal and Grassland Research and Innovation Centre, | ||
| 26 | Moorepark, Fermoy, Co. Cork).", | 26 | Moorepark, Fermoy, Co. Cork).", | ||
| 27 | "groups": [], | 27 | "groups": [], | ||
| 28 | "high_value_data": "false", | 28 | "high_value_data": "false", | ||
| 29 | "id": "ab5c94fe-4fb5-4140-8479-6f7da7645010", | 29 | "id": "ab5c94fe-4fb5-4140-8479-6f7da7645010", | ||
| 30 | "if_not_open_why": "", | 30 | "if_not_open_why": "", | ||
| 31 | "isopen": false, | 31 | "isopen": false, | ||
| 32 | "language": "en", | 32 | "language": "en", | ||
| 33 | "license_id": "notspecified", | 33 | "license_id": "notspecified", | ||
| 34 | "license_title": "Licence Not Specified", | 34 | "license_title": "Licence Not Specified", | ||
| 35 | "maintainer": null, | 35 | "maintainer": null, | ||
| 36 | "maintainer_email": null, | 36 | "maintainer_email": null, | ||
| 37 | "metadata_created": "2026-10-02T15:44:19.259795", | 37 | "metadata_created": "2026-10-02T15:44:19.259795", | ||
| n | 38 | "metadata_modified": "2026-10-02T15:44:19.259806", | n | 38 | "metadata_modified": "2026-10-02T15:52:27.377800", |
| 39 | "name": "bovine-colostrum-raw-sequence-reads", | 39 | "name": "bovine-colostrum-raw-sequence-reads", | ||
| 40 | "notes_translated": { | 40 | "notes_translated": { | ||
| 41 | "en": "16S rRNA gene (V4 region) amplicon sequencing data and | 41 | "en": "16S rRNA gene (V4 region) amplicon sequencing data and | ||
| 42 | associated colostrum quality data from 21 processed colostrum samples | 42 | associated colostrum quality data from 21 processed colostrum samples | ||
| 43 | collected immediately before calf feeding on a single spring-calving | 43 | collected immediately before calf feeding on a single spring-calving | ||
| 44 | Holstein-Friesian and Jersey dairy herd (Dairygold Research Farm, | 44 | Holstein-Friesian and Jersey dairy herd (Dairygold Research Farm, | ||
| 45 | Kilworth, Co. Cork) during the spring 2022 calving season. Colostrum | 45 | Kilworth, Co. Cork) during the spring 2022 calving season. Colostrum | ||
| 46 | from 31 primiparous and multiparous donors was refrigerated at 4 C for | 46 | from 31 primiparous and multiparous donors was refrigerated at 4 C for | ||
| 47 | up to 24 h, combined with a second donor's colostrum where needed (no | 47 | up to 24 h, combined with a second donor's colostrum where needed (no | ||
| 48 | more than two donors per sample), and reheated to 38 C in a water bath | 48 | more than two donors per sample), and reheated to 38 C in a water bath | ||
| 49 | for 60 min before sampling. The dataset characterises the prokaryotic | 49 | for 60 min before sampling. The dataset characterises the prokaryotic | ||
| 50 | community of colostrum as it is commonly managed on commercial dairy | 50 | community of colostrum as it is commonly managed on commercial dairy | ||
| 51 | farms, and complements an earlier dataset on fresh colostrum from the | 51 | farms, and complements an earlier dataset on fresh colostrum from the | ||
| 52 | same herd and calving season. Colostral immunoglobulin (Ig A, Ig G, Ig | 52 | same herd and calving season. Colostral immunoglobulin (Ig A, Ig G, Ig | ||
| 53 | M) concentrations were measured by single radial immunodiffusion. | 53 | M) concentrations were measured by single radial immunodiffusion. | ||
| 54 | Breed and parity had no effect on alpha- or beta-diversity; the | 54 | Breed and parity had no effect on alpha- or beta-diversity; the | ||
| 55 | community was low in diversity, heterogeneous across samples, and | 55 | community was low in diversity, heterogeneous across samples, and | ||
| 56 | dominated by Pseudomonas and Acinetobacter (bacteria) and | 56 | dominated by Pseudomonas and Acinetobacter (bacteria) and | ||
| 57 | Methanobrevibacter (archaea).\r\n", | 57 | Methanobrevibacter (archaea).\r\n", | ||
| 58 | "ga": "" | 58 | "ga": "" | ||
| 59 | }, | 59 | }, | ||
| 60 | "num_resources": 0, | 60 | "num_resources": 0, | ||
| 61 | "num_tags": 4, | 61 | "num_tags": 4, | ||
| 62 | "open_data": "Open data", | 62 | "open_data": "Open data", | ||
| 63 | "orcid_id": "https://orcid.org/0000-0001-9204-098X", | 63 | "orcid_id": "https://orcid.org/0000-0001-9204-098X", | ||
| 64 | "organization": { | 64 | "organization": { | ||
| 65 | "approval_status": "approved", | 65 | "approval_status": "approved", | ||
| 66 | "created": "2025-09-12T14:20:31.985845", | 66 | "created": "2025-09-12T14:20:31.985845", | ||
| 67 | "description": "", | 67 | "description": "", | ||
| 68 | "id": "b16b4c0b-15bd-4654-b227-792210c7076b", | 68 | "id": "b16b4c0b-15bd-4654-b227-792210c7076b", | ||
| 69 | "image_url": "", | 69 | "image_url": "", | ||
| 70 | "is_organization": true, | 70 | "is_organization": true, | ||
| 71 | "name": "animal-and-bioscience-research", | 71 | "name": "animal-and-bioscience-research", | ||
| 72 | "state": "active", | 72 | "state": "active", | ||
| 73 | "title": "Animal and Bioscience Research", | 73 | "title": "Animal and Bioscience Research", | ||
| 74 | "type": "organization" | 74 | "type": "organization" | ||
| 75 | }, | 75 | }, | ||
| 76 | "owner_org": "b16b4c0b-15bd-4654-b227-792210c7076b", | 76 | "owner_org": "b16b4c0b-15bd-4654-b227-792210c7076b", | ||
| 77 | "personal_data_flag": "false", | 77 | "personal_data_flag": "false", | ||
| 78 | "personal_sensitive_data": "false", | 78 | "personal_sensitive_data": "false", | ||
| 79 | "private": false, | 79 | "private": false, | ||
| 80 | "provenance": "Colostrum was collected from each quarter within 2-6 | 80 | "provenance": "Colostrum was collected from each quarter within 2-6 | ||
| 81 | h of calving using a portable milking unit, following sterile | 81 | h of calving using a portable milking unit, following sterile | ||
| 82 | microbiome sampling procedures (equipment cleaned with antimicrobial | 82 | microbiome sampling procedures (equipment cleaned with antimicrobial | ||
| 83 | soap and boiling water, teats and clusters disinfected with 70% | 83 | soap and boiling water, teats and clusters disinfected with 70% | ||
| 84 | ethanol). Each donor's colostrum was stored separately in sterile | 84 | ethanol). Each donor's colostrum was stored separately in sterile | ||
| 85 | buckets at 4 C for no more than 24 h, combined with a second donor | 85 | buckets at 4 C for no more than 24 h, combined with a second donor | ||
| 86 | where needed, and reheated to 38 C in a water bath for 60 min. Calves | 86 | where needed, and reheated to 38 C in a water bath for 60 min. Calves | ||
| 87 | (n = 21) did not receive colostrum from their own dam and were fed | 87 | (n = 21) did not receive colostrum from their own dam and were fed | ||
| 88 | 8.5% of birthweight within 2 h of birth. Immediately before feeding, | 88 | 8.5% of birthweight within 2 h of birth. Immediately before feeding, | ||
| 89 | 30 mL was collected aseptically into three sterile 10 mL tubes, snap | 89 | 30 mL was collected aseptically into three sterile 10 mL tubes, snap | ||
| 90 | frozen in liquid nitrogen and stored at -80 C. Samples were thawed | 90 | frozen in liquid nitrogen and stored at -80 C. Samples were thawed | ||
| 91 | stepwise (-20 C for 24 h, then 4 C for 24 h), treated with EDTA and | 91 | stepwise (-20 C for 24 h, then 4 C for 24 h), treated with EDTA and | ||
| 92 | centrifuged (4,500 x g, 20 min, 4 C). The fat layer was discarded, the | 92 | centrifuged (4,500 x g, 20 min, 4 C). The fat layer was discarded, the | ||
| 93 | supernatant kept for sRID assays, and the microbial pellet used for | 93 | supernatant kept for sRID assays, and the microbial pellet used for | ||
| 94 | DNA extraction (Qiagen DNeasy PowerSoil Pro Kit, repeated bead | 94 | DNA extraction (Qiagen DNeasy PowerSoil Pro Kit, repeated bead | ||
| 95 | beating). Bacterial DNA presence was confirmed by qPCR (Kittelmann et | 95 | beating). Bacterial DNA presence was confirmed by qPCR (Kittelmann et | ||
| 96 | al. 2013 method). Library preparation and sequencing were performed by | 96 | al. 2013 method). Library preparation and sequencing were performed by | ||
| 97 | Macrogen (Seoul, South Korea): V4 region amplified with 515F/806R | 97 | Macrogen (Seoul, South Korea): V4 region amplified with 515F/806R | ||
| 98 | primers carrying Nextera overhang adapters, 25 PCR cycles, AMPure | 98 | primers carrying Nextera overhang adapters, 25 PCR cycles, AMPure | ||
| 99 | purification, sequenced on an Illumina MiSeq with the 500-cycle v2 | 99 | purification, sequenced on an Illumina MiSeq with the 500-cycle v2 | ||
| 100 | reagent kit on one flow cell. Herd management: cows and heifers | 100 | reagent kit on one flow cell. Herd management: cows and heifers | ||
| 101 | grazed perennial ryegrass/white clover until November, then were | 101 | grazed perennial ryegrass/white clover until November, then were | ||
| 102 | housed on grass silage ad libitum without concentrate. Dry-off | 102 | housed on grass silage ad libitum without concentrate. Dry-off | ||
| 103 | treatments included parasite control and teat sealant for multiparous | 103 | treatments included parasite control and teat sealant for multiparous | ||
| 104 | cows; 11 multiparous cows with SCC > 100,000 also received | 104 | cows; 11 multiparous cows with SCC > 100,000 also received | ||
| 105 | intramammary antibiotic. Full protocols: Scully et al. (2026) PLOS | 105 | intramammary antibiotic. Full protocols: Scully et al. (2026) PLOS | ||
| 106 | ONE 21(7), e0353693, and Scully et al. (2025) PLOS ONE 20(10), | 106 | ONE 21(7), e0353693, and Scully et al. (2025) PLOS ONE 20(10), | ||
| 107 | e0335718.", | 107 | e0335718.", | ||
| 108 | "publish_to_public": true, | 108 | "publish_to_public": true, | ||
| 109 | "related_resource": [ | 109 | "related_resource": [ | ||
| 110 | "Scully, S., Earley, B., Smith, P. E., Finnie, M. S. J., McAloon, | 110 | "Scully, S., Earley, B., Smith, P. E., Finnie, M. S. J., McAloon, | ||
| 111 | C., Kenny, D. A. & Waters, S. M. (2026). Characterisation of the | 111 | C., Kenny, D. A. & Waters, S. M. (2026). Characterisation of the | ||
| 112 | bacterial and archaeal microbiota in processed colostrum collected | 112 | bacterial and archaeal microbiota in processed colostrum collected | ||
| 113 | from a spring-calving dairy herd. PLOS ONE 21(7), e0353693. | 113 | from a spring-calving dairy herd. PLOS ONE 21(7), e0353693. | ||
| 114 | https://doi.org/10.1371/journal.pone.0353693" | 114 | https://doi.org/10.1371/journal.pone.0353693" | ||
| 115 | ], | 115 | ], | ||
| 116 | "relationships_as_object": [], | 116 | "relationships_as_object": [], | ||
| 117 | "relationships_as_subject": [], | 117 | "relationships_as_subject": [], | ||
| 118 | "resources": [], | 118 | "resources": [], | ||
| 119 | "rights": "", | 119 | "rights": "", | ||
| 120 | "spatial": "", | 120 | "spatial": "", | ||
| 121 | "spatial_resolution": "", | 121 | "spatial_resolution": "", | ||
| 122 | "spatial_uri": "", | 122 | "spatial_uri": "", | ||
| 123 | "srs": [], | 123 | "srs": [], | ||
| t | 124 | "state": "approval_needed", | t | 124 | "state": "active", |
| 125 | "supplementary_data": "", | 125 | "supplementary_data": "", | ||
| 126 | "tags": [ | 126 | "tags": [ | ||
| 127 | { | 127 | { | ||
| 128 | "display_name": "Colostrum", | 128 | "display_name": "Colostrum", | ||
| 129 | "id": "4876f8bf-6c37-4d11-9116-8a33b8b009c4", | 129 | "id": "4876f8bf-6c37-4d11-9116-8a33b8b009c4", | ||
| 130 | "name": "Colostrum", | 130 | "name": "Colostrum", | ||
| 131 | "state": "active", | 131 | "state": "active", | ||
| 132 | "vocabulary_id": null | 132 | "vocabulary_id": null | ||
| 133 | }, | 133 | }, | ||
| 134 | { | 134 | { | ||
| 135 | "display_name": "Dairy farming", | 135 | "display_name": "Dairy farming", | ||
| 136 | "id": "b81d303f-e320-45a8-8f86-81e6365d5f7a", | 136 | "id": "b81d303f-e320-45a8-8f86-81e6365d5f7a", | ||
| 137 | "name": "Dairy farming", | 137 | "name": "Dairy farming", | ||
| 138 | "state": "active", | 138 | "state": "active", | ||
| 139 | "vocabulary_id": null | 139 | "vocabulary_id": null | ||
| 140 | }, | 140 | }, | ||
| 141 | { | 141 | { | ||
| 142 | "display_name": "Gene sequencing", | 142 | "display_name": "Gene sequencing", | ||
| 143 | "id": "06cd453c-1d85-41ea-bd6b-b2add99540dd", | 143 | "id": "06cd453c-1d85-41ea-bd6b-b2add99540dd", | ||
| 144 | "name": "Gene sequencing", | 144 | "name": "Gene sequencing", | ||
| 145 | "state": "active", | 145 | "state": "active", | ||
| 146 | "vocabulary_id": null | 146 | "vocabulary_id": null | ||
| 147 | }, | 147 | }, | ||
| 148 | { | 148 | { | ||
| 149 | "display_name": "Microbiome", | 149 | "display_name": "Microbiome", | ||
| 150 | "id": "7576d3c7-4914-4113-b5c1-b1a9e95c1436", | 150 | "id": "7576d3c7-4914-4113-b5c1-b1a9e95c1436", | ||
| 151 | "name": "Microbiome", | 151 | "name": "Microbiome", | ||
| 152 | "state": "active", | 152 | "state": "active", | ||
| 153 | "vocabulary_id": null | 153 | "vocabulary_id": null | ||
| 154 | } | 154 | } | ||
| 155 | ], | 155 | ], | ||
| 156 | "temporal": "January-March 2022", | 156 | "temporal": "January-March 2022", | ||
| 157 | "theme": "animal_and_grassland", | 157 | "theme": "animal_and_grassland", | ||
| 158 | "title": "Bovine Colostrum Raw sequence reads", | 158 | "title": "Bovine Colostrum Raw sequence reads", | ||
| 159 | "title_translated": { | 159 | "title_translated": { | ||
| 160 | "en": "Bovine Colostrum Raw sequence reads", | 160 | "en": "Bovine Colostrum Raw sequence reads", | ||
| 161 | "ga": "" | 161 | "ga": "" | ||
| 162 | }, | 162 | }, | ||
| 163 | "type": "dataset", | 163 | "type": "dataset", | ||
| 164 | "update_frequency": "", | 164 | "update_frequency": "", | ||
| 165 | "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1136803/", | 165 | "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1136803/", | ||
| 166 | "version": "", | 166 | "version": "", | ||
| 167 | "vertical_extent": "" | 167 | "vertical_extent": "" | ||
| 168 | } | 168 | } |
