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On 5 Deireadh Fómhair 2026 16:28:07 UTC,
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todheeraj.rathore@teagasc.ie
in Fungal microbiome structure across phyllosphere compartments of Eucalyptus cinerea
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| 5 | "author": null, | 5 | "author": null, | ||
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| n | 10 | "contact_email": "https://orcid.org/0000-0003-3787-6084", | n | 10 | "contact_email": "dheeraj.rathore@teagasc.ie", |
| 11 | "contact_name": "Dr Dheeraj Singh Rathore", | 11 | "contact_name": "Dr Dheeraj Singh Rathore", | ||
| 12 | "creator_user_id": "68d970d0-ff26-4dfe-ab51-46dde87e3f23", | 12 | "creator_user_id": "68d970d0-ff26-4dfe-ab51-46dde87e3f23", | ||
| 13 | "data_creator": [ | 13 | "data_creator": [ | ||
| 14 | "Tom\u00e1s Byrne" | 14 | "Tom\u00e1s Byrne" | ||
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| 16 | "doi": "", | 16 | "doi": "", | ||
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| 25 | "language": "en", | 25 | "language": "en", | ||
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| 27 | "license_title": "Licence Not Specified", | 27 | "license_title": "Licence Not Specified", | ||
| 28 | "maintainer": null, | 28 | "maintainer": null, | ||
| 29 | "maintainer_email": null, | 29 | "maintainer_email": null, | ||
| 30 | "metadata_created": "2026-10-05T16:27:20.453963", | 30 | "metadata_created": "2026-10-05T16:27:20.453963", | ||
| n | 31 | "metadata_modified": "2026-10-05T16:27:20.453974", | n | 31 | "metadata_modified": "2026-10-05T16:28:07.726468", |
| 32 | "name": | 32 | "name": | ||
| 33 | ome-structure-across-phyllosphere-compartments-of-eucalyptus-cinerea", | 33 | ome-structure-across-phyllosphere-compartments-of-eucalyptus-cinerea", | ||
| 34 | "notes_translated": { | 34 | "notes_translated": { | ||
| 35 | "en": "Fungal ITS amplicon sequencing data (Illumina NovaSeq, 2 x | 35 | "en": "Fungal ITS amplicon sequencing data (Illumina NovaSeq, 2 x | ||
| 36 | 150 bp) from leaf, stem and bark tissues of five Eucalyptus cinerea | 36 | 150 bp) from leaf, stem and bark tissues of five Eucalyptus cinerea | ||
| 37 | trees in an intensively managed commercial cut foliage plantation in | 37 | trees in an intensively managed commercial cut foliage plantation in | ||
| 38 | Co. Dublin, sampled on one day in July 2024. Tissues were pooled by | 38 | Co. Dublin, sampled on one day in July 2024. Tissues were pooled by | ||
| 39 | compartment within each tree, giving 15 samples (5 trees x 3 | 39 | compartment within each tree, giving 15 samples (5 trees x 3 | ||
| 40 | compartments). Tissues were not surface-sterilised, so the profiles | 40 | compartments). Tissues were not surface-sterilised, so the profiles | ||
| 41 | combine epiphytic and endophytic fungi. After quality control and | 41 | combine epiphytic and endophytic fungi. After quality control and | ||
| 42 | DADA2 denoising, 405 fungal ASVs were retained. Bark carried richer, | 42 | DADA2 denoising, 405 fungal ASVs were retained. Bark carried richer, | ||
| 43 | more diverse and phylogenetically broader fungal communities than leaf | 43 | more diverse and phylogenetically broader fungal communities than leaf | ||
| 44 | and stem: bark was dominated by Diaporthe, Peniophora and Talaromyces, | 44 | and stem: bark was dominated by Diaporthe, Peniophora and Talaromyces, | ||
| 45 | and leaf and stem by Vishniacozyma and Sporobolomyces. Both | 45 | and leaf and stem by Vishniacozyma and Sporobolomyces. Both | ||
| 46 | compartment (PERMANOVA R2 = 0.239) and tree identity (R2 = 0.451) | 46 | compartment (PERMANOVA R2 = 0.239) and tree identity (R2 = 0.451) | ||
| 47 | significantly affected community composition. The dataset is a | 47 | significantly affected community composition. The dataset is a | ||
| 48 | baseline description of the fungal microbiome of E. cinerea grown | 48 | baseline description of the fungal microbiome of E. cinerea grown | ||
| 49 | commercially under Irish conditions.", | 49 | commercially under Irish conditions.", | ||
| 50 | "ga": "" | 50 | "ga": "" | ||
| 51 | }, | 51 | }, | ||
| 52 | "num_resources": 0, | 52 | "num_resources": 0, | ||
| 53 | "num_tags": 5, | 53 | "num_tags": 5, | ||
| 54 | "open_data": "Open data", | 54 | "open_data": "Open data", | ||
| 55 | "orcid_id": "https://orcid.org/0000-0003-4931-7263", | 55 | "orcid_id": "https://orcid.org/0000-0003-4931-7263", | ||
| 56 | "organization": { | 56 | "organization": { | ||
| 57 | "approval_status": "approved", | 57 | "approval_status": "approved", | ||
| 58 | "created": "2026-10-05T15:46:06.560119", | 58 | "created": "2026-10-05T15:46:06.560119", | ||
| 59 | "description": "", | 59 | "description": "", | ||
| 60 | "id": "082abffe-7eb8-4f8b-a78e-a664136a1a39", | 60 | "id": "082abffe-7eb8-4f8b-a78e-a664136a1a39", | ||
| 61 | "image_url": "", | 61 | "image_url": "", | ||
| 62 | "is_organization": true, | 62 | "is_organization": true, | ||
| 63 | "name": "forestry-development", | 63 | "name": "forestry-development", | ||
| 64 | "state": "active", | 64 | "state": "active", | ||
| 65 | "title": "Forestry Development", | 65 | "title": "Forestry Development", | ||
| 66 | "type": "organization" | 66 | "type": "organization" | ||
| 67 | }, | 67 | }, | ||
| 68 | "owner_org": "082abffe-7eb8-4f8b-a78e-a664136a1a39", | 68 | "owner_org": "082abffe-7eb8-4f8b-a78e-a664136a1a39", | ||
| 69 | "personal_data_flag": "false", | 69 | "personal_data_flag": "false", | ||
| 70 | "personal_sensitive_data": "false", | 70 | "personal_sensitive_data": "false", | ||
| 71 | "private": false, | 71 | "private": false, | ||
| 72 | "provenance": "A commercial E. cinerea cut foliage plantation in Co. | 72 | "provenance": "A commercial E. cinerea cut foliage plantation in Co. | ||
| 73 | Dublin, established in 2019 and managed intensively (irrigation, | 73 | Dublin, established in 2019 and managed intensively (irrigation, | ||
| 74 | fertilisation, herbicide weed control). Five trees of similar age and | 74 | fertilisation, herbicide weed control). Five trees of similar age and | ||
| 75 | management history were selected at random across three production | 75 | management history were selected at random across three production | ||
| 76 | fields. From each tree, six leaves, six stem sections (about 2 cm) and | 76 | fields. From each tree, six leaves, six stem sections (about 2 cm) and | ||
| 77 | six bark pieces were taken from several canopy positions and pooled by | 77 | six bark pieces were taken from several canopy positions and pooled by | ||
| 78 | compartment. All samples were collected on one day, put on ice, and | 78 | compartment. All samples were collected on one day, put on ice, and | ||
| 79 | stored at -80 C. Tissues were not surface-sterilised. DNA and | 79 | stored at -80 C. Tissues were not surface-sterilised. DNA and | ||
| 80 | sequencing: total DNA was extracted with the Qiagen DNeasy Plant Mini | 80 | sequencing: total DNA was extracted with the Qiagen DNeasy Plant Mini | ||
| 81 | Kit. Novogene (Cambridge, UK) amplified the fungal ITS region with | 81 | Kit. Novogene (Cambridge, UK) amplified the fungal ITS region with | ||
| 82 | primers ITS5-1737F (5'-GGAAGTAAAAGTCGTAACAAGG-3') and ITS2-2043R | 82 | primers ITS5-1737F (5'-GGAAGTAAAAGTCGTAACAAGG-3') and ITS2-2043R | ||
| 83 | (5'-GCTGGTTCATTCATCGATGC-3'), prepared libraries, and sequenced them | 83 | (5'-GCTGGTTCATTCATCGATGC-3'), prepared libraries, and sequenced them | ||
| 84 | on an Illumina NovaSeq (PE150). Library negative controls produced no | 84 | on an Illumina NovaSeq (PE150). Library negative controls produced no | ||
| 85 | detectable amplification and were not sequenced.", | 85 | detectable amplification and were not sequenced.", | ||
| 86 | "publish_to_public": true, | 86 | "publish_to_public": true, | ||
| 87 | "related_resource": [ | 87 | "related_resource": [ | ||
| 88 | "Byrne, T. & Rathore, D. S. (2026). Fungal Microbiome Structure | 88 | "Byrne, T. & Rathore, D. S. (2026). Fungal Microbiome Structure | ||
| 89 | Across Phyllosphere Compartments in Intensively Managed Eucalyptus | 89 | Across Phyllosphere Compartments in Intensively Managed Eucalyptus | ||
| 90 | cinerea for Cut Foliage Production. Applied Microbiology 6(7), 76. | 90 | cinerea for Cut Foliage Production. Applied Microbiology 6(7), 76. | ||
| 91 | https://doi.org/10.3390/applmicrobiol6070076", | 91 | https://doi.org/10.3390/applmicrobiol6070076", | ||
| 92 | "Bioinformatic workflows and analysis scripts are available on | 92 | "Bioinformatic workflows and analysis scripts are available on | ||
| 93 | GitHub at: | 93 | GitHub at: | ||
| 94 | https://github.com/ByrneTforestry/its-fungal-amplicon-Eucalyptus" | 94 | https://github.com/ByrneTforestry/its-fungal-amplicon-Eucalyptus" | ||
| 95 | ], | 95 | ], | ||
| 96 | "relationships_as_object": [], | 96 | "relationships_as_object": [], | ||
| 97 | "relationships_as_subject": [], | 97 | "relationships_as_subject": [], | ||
| 98 | "resources": [], | 98 | "resources": [], | ||
| 99 | "rights": "", | 99 | "rights": "", | ||
| 100 | "spatial": "", | 100 | "spatial": "", | ||
| 101 | "spatial_resolution": "", | 101 | "spatial_resolution": "", | ||
| 102 | "spatial_uri": "", | 102 | "spatial_uri": "", | ||
| 103 | "srs": [], | 103 | "srs": [], | ||
| t | 104 | "state": "approval_needed", | t | 104 | "state": "active", |
| 105 | "supplementary_data": "", | 105 | "supplementary_data": "", | ||
| 106 | "tags": [ | 106 | "tags": [ | ||
| 107 | { | 107 | { | ||
| 108 | "display_name": "Eucalyptus cinerea", | 108 | "display_name": "Eucalyptus cinerea", | ||
| 109 | "id": "bacf9838-9611-47be-840f-9c9dcdf72adc", | 109 | "id": "bacf9838-9611-47be-840f-9c9dcdf72adc", | ||
| 110 | "name": "Eucalyptus cinerea", | 110 | "name": "Eucalyptus cinerea", | ||
| 111 | "state": "active", | 111 | "state": "active", | ||
| 112 | "vocabulary_id": null | 112 | "vocabulary_id": null | ||
| 113 | }, | 113 | }, | ||
| 114 | { | 114 | { | ||
| 115 | "display_name": "Foliage", | 115 | "display_name": "Foliage", | ||
| 116 | "id": "591e0846-195e-4416-bbbd-a71f533a3304", | 116 | "id": "591e0846-195e-4416-bbbd-a71f533a3304", | ||
| 117 | "name": "Foliage", | 117 | "name": "Foliage", | ||
| 118 | "state": "active", | 118 | "state": "active", | ||
| 119 | "vocabulary_id": null | 119 | "vocabulary_id": null | ||
| 120 | }, | 120 | }, | ||
| 121 | { | 121 | { | ||
| 122 | "display_name": "Mycobiome", | 122 | "display_name": "Mycobiome", | ||
| 123 | "id": "6c0be5a8-904b-427d-b535-3719e410662e", | 123 | "id": "6c0be5a8-904b-427d-b535-3719e410662e", | ||
| 124 | "name": "Mycobiome", | 124 | "name": "Mycobiome", | ||
| 125 | "state": "active", | 125 | "state": "active", | ||
| 126 | "vocabulary_id": null | 126 | "vocabulary_id": null | ||
| 127 | }, | 127 | }, | ||
| 128 | { | 128 | { | ||
| 129 | "display_name": "Phyllosphere microbiome", | 129 | "display_name": "Phyllosphere microbiome", | ||
| 130 | "id": "31ec592a-870c-4742-a602-a6cf5af5261a", | 130 | "id": "31ec592a-870c-4742-a602-a6cf5af5261a", | ||
| 131 | "name": "Phyllosphere microbiome", | 131 | "name": "Phyllosphere microbiome", | ||
| 132 | "state": "active", | 132 | "state": "active", | ||
| 133 | "vocabulary_id": null | 133 | "vocabulary_id": null | ||
| 134 | }, | 134 | }, | ||
| 135 | { | 135 | { | ||
| 136 | "display_name": "Sequencing", | 136 | "display_name": "Sequencing", | ||
| 137 | "id": "1883e57e-6a22-4b18-a0ce-47d8e973141c", | 137 | "id": "1883e57e-6a22-4b18-a0ce-47d8e973141c", | ||
| 138 | "name": "Sequencing", | 138 | "name": "Sequencing", | ||
| 139 | "state": "active", | 139 | "state": "active", | ||
| 140 | "vocabulary_id": null | 140 | "vocabulary_id": null | ||
| 141 | } | 141 | } | ||
| 142 | ], | 142 | ], | ||
| 143 | "temporal": "July 2024", | 143 | "temporal": "July 2024", | ||
| 144 | "theme": "crops_environment_and_land_use", | 144 | "theme": "crops_environment_and_land_use", | ||
| 145 | "title": " Fungal microbiome structure across phyllosphere | 145 | "title": " Fungal microbiome structure across phyllosphere | ||
| 146 | compartments of Eucalyptus cinerea", | 146 | compartments of Eucalyptus cinerea", | ||
| 147 | "title_translated": { | 147 | "title_translated": { | ||
| 148 | "en": " Fungal microbiome structure across phyllosphere | 148 | "en": " Fungal microbiome structure across phyllosphere | ||
| 149 | compartments of Eucalyptus cinerea", | 149 | compartments of Eucalyptus cinerea", | ||
| 150 | "ga": "" | 150 | "ga": "" | ||
| 151 | }, | 151 | }, | ||
| 152 | "type": "dataset", | 152 | "type": "dataset", | ||
| 153 | "update_frequency": "", | 153 | "update_frequency": "", | ||
| 154 | "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1460758/ ", | 154 | "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1460758/ ", | ||
| 155 | "version": "", | 155 | "version": "", | ||
| 156 | "vertical_extent": "" | 156 | "vertical_extent": "" | ||
| 157 | } | 157 | } |
